Kicking Horse Bighorn Sheep Project
/Golden and District Rod and Gun Club
Kicking Horse Bighorn Sheep Project
(Health and Movement Data Summary
March 27, 2019 to November 30, 2020)
Prepared by:
Jeremy Ayotte MSc, RPBio Phyla Biological Consulting Inc.
Prepared for:
Golden and District Rod and Gun Club
Summary of bighorn sheep movement and health data to November 30, 2020
- 5 Survey Iridium GPS collars were purchased from Vectronic Aerospace and designed for deployment on bighorn sheep. Collars were programmed to record locations every 4-6 hours and provide mortality alerts if a collar has not moved in 12 hours. High fix rates and long battery life are trade-offs, the average battery lifetime estimate for these collars is 4.2 years 
- All 5 GPS collars have now been deployed: - 2 ewes were captured, sampled and collared 27 March 2019 
- 1 ram was captured, sampled and collared 14 May 2019 
- 2 rams were captured, sampled and collared 14 May 2020 
 
- Chemical immobilization was administered using a dart gun from a vehicle or on foot at a distance of less than 30 m. BAM II (butorphanol/azaperone/medetomidine) was injected using 1.5 cc Pneu-Darts (tri-port 14 guage wire barb) delivered into a large hindquarter muscle. Following completion of sampling and collaring, a reversal of atipamezole and naltrexone was subcutaneously injected into the hind quarters 
- Using standard Ministry FLNRORD Wildlife Health Program sample kits, health samples were collected from all 5 bighorns and included nasal swabs, ear swabs, blood, hair, fecal, and DNA samples 
- Lab results from nasal swabs confirm that here has been no Mycoplasma ovipneumoniae detected in nasal swabs or blood serum samples. M.ovi is a respiratory bacteria spread from domestic to wild sheep and is a primary concern in health and conservation of wild sheep. We are currently waiting on lab results from the 2 rams collared 14 May 2020 
- Both ewes were pregnant at the time of capture. 
- DNA samples (ear punch) will be used to investigate a genomic estimate of inbreeding for this population, and will also contribute to a larger study on the genetic analysis of California vs Rocky Mountain bighorn. Results pending. 
- Fecal pellets were analyzed for cortisol levels as a stress indicator. 
All health sample results will be archived in the Ministry of FLNRORD Wildlife Health Database
- GPS collars have collected over 10,000 locations from 5 collared bighorn sheep: - Ewe 34087: 3144 locations 
- Ewe 34091: 3426 locations 
- Ram 36002: 2275 locations 
- Ram 36003: 771 locations 
- Ram 36006: 745 locations 
 
25% (2559/10361) of all collared sheep locations were within the KHCP project limits. Female bighorn sheep spend over 30% of their time within the project limits. The breakdown by individual sheep is:
- Initial investigation of lambing sites (10 May to 4 June, from Poole 2013) for the 2 collared bighorn ewes, suggests that there are potential lambing sites within the project limits (Fig. 3). All ewe locations during this sensitive period are within 1 km of the Project Limits. Field verification is required to confirm that these ewes actually had a lamb at heel. 

 
                     
             
             
             
             
            